Fig. 3
BMP8b-dependent regulation of NRG4 in AT and adipocytes. a, b Fold variation of mRNA levels of molecules that regulate sympathetic neuron survival, or axonal or dendritic guidance or growth in BAT (a) and ScW (b) from 5-week-old WT and Bmp8b TG female mice; n = 8–9. c NRG4 mRNA levels in BAT and ScW from 4-week-old WT and TG female mice born and housed at 28 °C, n = 7–8. Data are represented normalized to the WT value; *p < 0.05 compared to WT using Student’s t-test (e–h). d Fold variation of NRG4 mRNA levels in differentiated brown adipocytes chronically (9 days, during differentiation) or acutely (24 h at day 8 of differentiation) treated with human recombinant BMP8 (red bar and pink bar, respectively). e–g ELISA analysis of NRG4 secretion of white unilocular (isolated from ScW) (e) and primary brown differentiated adipocytes (isolated from BAT) from 10-week-old Bmp8b TG versus WT (e, f) and Bmp8b+/+versus Bmp8b–/– (g) mice; n = 3–5. Mean ± s.e.m. are presented; *p < 0.05, compared to WT using multiple t-test with FDR determined using the two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli (a, b); *p < 0.05, **p<0.01 compared to WT using Mann–Whitney test (c, e, f); *p < 0.05, one-way ANOVA compared to control with Sidak’s post-hoc multiple comparisons test (d)
