Fig. 4
NRG4 promoted primary sympathetic neuron axonal growth and branching. a Representative images of P0 primary sympathetic neurons cultured for 24 h without factors (control) or with NRG4 or NGF, Scale bar, 50 µm. b Quantification of axon length and number of branch points in SCG neuron arbors at stage E14 to P5 cultured without factors or with either NRG4 or NGF. c Axon growth and branching dose responses of P0 SCG neurons to NRG4. d Axon growth and branching dose responses of P0 SCG neurons to NGF with and without 1 μg/ml NRG4. Mean ± s.e.m of data of >150 neurons per condition combined from 3 experiments. Mean ± s.e.m. are presented. *P < 0.05, **p < 0.01, ***p < 0.001 compared to 0 ng/ml NRG4 (c) and NGF conditions (d) using one-way (c) or two-way (d) ANOVA with Bonferroni–Holm post-hoc multiple comparison test
