Fig. 5
BMP8b overexpression induced remodeling of vasculature network. a, b Representative images of confocal analysis of Isolectin IB4 staining in BAT and ScW from 5-week-old WT and Bmp8b TG mice (a) and quantification of % of positive area (b). Scale bar, 100 µm. c Quantification of the number of vessels (isolectin+, caveolin-1+) per adipocytes (caveolin-1+, isolectin-) based on the representative images in Supplementary Figure 5a; n = 3–6. d–f Fold variation of mRNA levels of ECs (d), pericyte (e) markers and angiogenic factor receptors (f) in ScW from 4-week-old Bmp8b TG female mice, n = 4–6. Data are expressed in fold variation over control (4-week-old WT littermate). g, h Fold variation of mRNA levels of pro-angiogenic factors, factors promoting the maturity of newly made capillaries (Angpt1, Angptl4) and anti-angiogenic factors (Thbs1,Cxcl10) in ScW (g) and BAT (h) from 4-week-old Bmp8b TG female mice, n = 4–6. Data are expressed in fold variation over control (4-week-old WT littermate). i, j Representative images of confocal analysis isolectin staining in BAT and ScW from 5-week-old WT and Bmp8b TG female mice born and housed at 28 °C (i) and quantification (j) of % area. Scale bar, 100 µm. Mean ± s.e.m. are presented. *P < 0.05, **p < 0.01, compared to WT using two-way ANOVA with Sidak’s post-hoc multiple comparisons test (b, c, j); *p < 0.05, **p<0.01, ***p<0.001, compared to WT using multiple t-test with FDR determined using the two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli (d–h)
