Fig. 6
BMP8b induces a pro-angiogenic profile in adipocytes. a Effects of recombinant BMP8 on MCEC vascular tube formation (n = 3–5 experiments). The data shown are representative of one experiment performed in triplicate. b VEGF protein level secreted from mature adipocytes isolated from ScW of 10-week-old WT or Bmp8b TG mice (24 h secretion); n = 4. c mRNA levels of angiogenic factors in white adipocytes treated or not with recombinant BMP8 (100pM) before (PA, 24) and during (AD, 9 days) differentiation; n = 4. d VEGF protein in serum of 10-week-old WT and Bmp8b TG mice; n = 4. e Heat map presenting fold variation of protein levels of pro/anti-angiogenic factors secreted from ScW and BAT explants from 10-week-old WT and TG mice. Data from one experiment of angiogenic proteome array (Supplementary Figure 8d) on a pool of three independent experiments are presented. f VEGF protein level secreted from ScW explants of 10-week-old WT or Bmp8b TG mice (24 h secretion); n = 3–4. g–i Representative images of 10-week-old WT and Bmp8b TG (TG) mice ScW explants (g) or BMP8-treated explants (i) cultured on Matrigel without (Control) or with NE stimulation (10–7 M) (g) and/or without (TG) or with (TG+SB431542) Alk7 antagonist (SB431542) for 10 days (h). Dotted orange lines encompass the explant; dotted red lines show the cell sprouting area. Quantification of cell sprouting area, n = 7–8. Scale bar, 500 µm. Mean ± s.e.m. are presented. *P < 0.05, **p<0.01, compared to 0 M BMP8 condition using one-way ANOVA with Sidak’s post-hoc multiple comparisons test (a, c, i), *p < 0.05, **p<0.01, compared to WT (g) or TG (h) using two-way ANOVA with Sidak’s post-hoc multiple comparisons test; *p < 0.05 compared to WT using a t-test (b)
