Fig. 7
Phophoproteomic array analysis of BMP8b signaling in brown adipocytes. Brown differentiated adipocytes were treated for 30 min with human recombinant BMP8 (100 pM) or NE (75 nM) or combination of both, prior to phosphoproteome analysis. a, b Venn diagrams showing proteins with fold change values above 2 (twice more (a) or half less (b) phosphorylated) on any of their phosphosites between treatments. Proteins are represented by standard human gene names. The number of proteins in each field is proportional with the area of the field and shown by numbers. a Positively regulated proteins according to fold change signs adjusted by the known effect signs. Phosphosites without known effect signs were not considered here and at (b). b Inhibited proteins with similar sign adjustment. c Heat map representing sign adjusted fold changes and functional annotations of the top 50 phosphosites in BMP8, NE and BMP8 and NE treated brown adipocytes. Cells in the leftmost frame show log2 fold change values with signs adjusted by their known effects. Green, white and red corresponds to inhibition, no change and stimulation, respectively. Clustering and dendrogram constructed with Euclidean distance and complete linkage. Sites for which no effect sign is known from PhosphpSitePlus are not shown here. On the middle frames are shown the functional annotations mapped from the Gene Ontology for the substrates (named as “self”), their kinases (name as “kinases”) and their downstream regulated interaction partners (named as “regulated”). Shades of gray of each cell correspond to the proportion of all interacting proteins annotated with at least one term in the group. A angiogenesis, I inflammation, L lipid metabolism and thermogenic activity, N neurogenesis, C cell cycle and survival
