Fig. 1
From: A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
Schematic representation of strategy for development of CL-GESS. ε-Caprolactam activates NitR derived from CL-GESS, which then activates the PnitA promoter and sfgfp gene expression in CL-GESS, resulting in fluorescence emission. a Schematic illustration of the screening procedure for engineering the NitR regulator by random mutagenesis. b Systematic combinatorial analysis and optimization of promoter and RBS for NitR expression. c Identification of the PnitA region by gene truncation. d Reporter change in CL-GESS
