Fig. 4
From: A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
HTS of cyclases from metagenome library using CL-GESSL117F. a Selection of a flow cytometry histogram from metagenome library containing 500 μM 6-ACA and selection of a strongly fluorescent colony (positive hit) on LB agar containing 10 mM 6-ACA. The scale bar is 5 mm. b Analysis of fluorescence in a clone with 11 ORFs in pET28a(+) from a metagenomic fosmid using CL-GESSL117F to assess ε-caprolactam-converting activity from 10 mM 6-ACA over 24 h. Values represent means ± SDs of three independent experiments
