Table 2 Specific activities of wild-type CF3HBD and mutant enzymes of the active site residues
From: A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
3-Hydroxybutyric acid | 6-Aminocaproic acid | |||
|---|---|---|---|---|
Specific activity (U/mg) | Relative activity (%) | Specific activity (U/mg) | Relative activity (%) | |
WT | 79.2 ± 2.5 | 100.0 ± 3.2 | 2.1 ± 0.0 | 100.0 ± 0.3 |
Q91A | –a | – | – | – |
Q91E | 90.9 ± 2.3 | 114.9 ± 2.9 | 2.9 ± 0.1 | 139.5 ± 6.4 |
S139A | – | – | – | – |
V140A | – | – | 1.7 ± 0.1 | 83.2 ± 4.3 |
H141A | – | – | – | – |
H141E | 0.9 ± 0.03 | 1.1 ± 0.04 | 1.2 ± 0.0 | 56.0 ± 0.7 |
K149A | 7.1 ± 0.4 | 8.9 ± 0.5 | 1.8 ± 0.1 | 88.0 ± 4.8 |
K149E | 23.5 ± 0.3 | 29.7 ± 0.4 | 1.6 ± 0.0 | 76.5 ± 5.0 |
Y152A | – | – | 6.5 ± 0.5 | 312.9 ± 23.2 |
Y152E | 28.5 ± 1.3 | 36.0 ± 1.6 | 2.1 ± 0.7 | 101.5 ± 3.5 |
W184A | – | – | 1.7 ± 0.2 | 84.2 ± 7.5 |
W184E | – | – | 1.8 ± 0.1 | 88.0 ± 4.0 |
W189A | 16.4 ± 0.5 | 20.8 ± 0.7 | 2.2 ± 0.0 | 106.6 ± 1.3 |
V190A | 77.7 ± 3.6 | 98.2 ± 4.6 | 1.8 ± 0.1 | 86.0 ± 4.7 |
Q193A | 14.6 ± 0.2 | 18.4 ± 0.3 | 1.0 ± 0.0 | 50.0 ± 1.8 |
Q193E | 168.1 ± 5.8 | 212.4 ± 7.4 | 2.6 ± 0.2 | 127.5 ± 7.2 |
