Table 3 Kinetic parameters of the wild-type and Y152A mutant enzymes toward 6-aminocaproic acid
From: A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
Wild type | Y152A | |
|---|---|---|
Km (mM) | 3.3 ± 0.3 | 2.9 ± 0.1 |
kcat (min−1 × 10−3) | 72.2 ± 2.0 | 208.5 ± 1.6 |
kcat/Km (min−1 M−1) | 21.8 ± 1.8 | 73.1 ± 2.1 |
