Fig. 5
Effect of PTPN1 mutation on energy metabolism alteration. a Trehalose level and glucose concentration in hemolymph and thoracic muscle. The relative ratio is the ratio of the peak area of metabolic intermediate to the peak area of the internal standard (sucrose). (n ≥ 6 replicates, 3 locusts/replicate). b The levels of glycogen, acetyl-CoA, and NADH in the thoracic muscle and lactate in hemolymph. Values in a, b are the mean ± s.e.m. Significant differences are denoted by letters (one-way ANOVA, P < 0.05) (n = 4 replicates, 3 locusts/replicate). c PTP1B knockdown affected hypoxia-induced metabolic regulation by the insulin pathway (n = 5 replicates, three locusts/replicate). *P < 0.05, **P < 0.01 (Student’s t test). N.S. represents no significant difference (Student’s t test). The center line of the boxplots represents median value, the bounds of the box represent 75th and 25th percentile, and the whiskers represent maximum and minimum value
