Table 1 FRAP fraction recovery and half recovery times of NPM1 in NPM1-S6N droplets with and without crowding agent

From: Compositional adaptability in NPM1-SURF6 scaffolding networks enabled by dynamic switching of phase separation mechanisms

Buffer

η buffer (mPa·s)

Mf

t1/2 (s)

Dapp (μm2·s−1)

0% PEG

1.0 ± 0.001

0.79 ± 0.001

14.3 ± 0.2

2.9 × 10-3 ± 4.0 × 10−5

5% PEG

2.4 ± 0.001

0.27 ± 0.003

22.5 ± 1.7

2.1 × 1 0-3 ± 1.3 × 0−4

15% PEG

10.7 ± 0.02

~0.05

ND

ND

  1. All solutions contain 10 mM Tris, 150 mM NaCl, 2 mM DTT, pH 7.5
  2. η  viscosity, Mf fraction of mobile NPM1 from FRAP, t1/2 half-time of fluorescence recovery,
  3. Dapp apparent diffusion coefficient, mean ± s.d.; n ≥ 9 FRAP measurements; n = 4 for buffer viscosity determination; ND cannot be determined from fitted FRAP curves
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