Fig. 1 | Nature Communications

Fig. 1

From: Single cell RNA-sequencing identifies a metabolic aspect of apoptosis in Rbf mutant

Fig. 1

A cell atlas of the wild-type third-instar larval eye disc. a A cartoon representation of the eye-antennal disc and known cell types that are color coded. The red vertical lines indicate where the dissections were made. The expression domains of genes above that were used to assign cell clusters. D dorsal, V ventral, P posterior to the MF, A anterior to the MF. b The pattern of the expression of cell-type markers in the eye as revealed by immunofluorescence. BrdU labeling shows the location of SMW (yellow arrow) and PG (yellow arrowhead) cells undergoing cell division. The scale bar is 50 µm. c The tSNE representation of 11,416 wild-type eye disc cells. Each cluster represents a cell type/domain, characterized by a unique gene expression signature. Clusters are color coded as in (a). Other cells are labeled as gray. d Gene/gene plots generated on all photoreceptors for sens (R8) and ro (R2/5), sens and B-H2 (R1/6). The percentage represents doublets, based on the detection of two R cell-specific markers in the same single-cell library. The position of the MF is shown by white arrowhead

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