Fig. 4
From: Single cell RNA-sequencing identifies a metabolic aspect of apoptosis in Rbf mutant
Photoreceptors undergo a transcriptional switch at onset of axonogenesis. a Gene/gene plots showing expression of R type-specific markers sens, ro and B-H2 in relation to elav in EPR (left panel) and LPR (right panel). b Venn diagram of the gene lists for EPR and LPR clusters. c Feature plots on tSNE for twit and Ace show predominant expression of each gene in EPR and LPR cells, respectively. d EPR and LPR marker gene localization. Left panel: FISH for twit mRNA, an EPR-specific gene, shows expression in the MF and posterior until column 4. Right panel: GFP enhancer trap for Ace (green), an LPR top marker, was costained with Sens (red). Ace-GFP was detected after column 4 and in axonal projections (yellow arrow). The scale bar is 50 µm. e Gene enrichment analysis for biological processes in UND, EPR and LPR. f Monocle 2 trajectory using shown clusters. Stages of differentiation are visualized, with the split of INT and photoreceptor populations. LPR arise from the EPR population, clustering consecutively in pseudotime. The position of the MF is shown by white arrowhead
