Fig. 6
From: Single cell RNA-sequencing identifies a metabolic aspect of apoptosis in Rbf mutant
Upregulation of Ldh, Ald and HIF1a make Rbf mutant prone to apoptosis. a Cleaved Dcp-1 immunofluorescence displays the distinct wave of apoptosis in Rbf120a that is rescued by downregulation of each Ldh, Ald and HIF1a. The scale bar is 50 µm. b Quantification of cleaved Dcp-1-positive puncta in N (WT) = 20 eyes, N (Rbf120a) = 62 eyes, N (Rbf120a, LdhRNAi) = 54 eyes, N (Rbf120a, AldRNAi) = 58 eyes, and N (Rbf120a, HIF1ARNAi) = 59 eyes. Center box line represents the median. The lower box limit is the first quartile. The upper box limit is the third quartile. The whiskers point at the variabilities outside the first and third quartiles. Values outside the whiskers are outliers. The hollow circles are outliers. One-way analysis of variance (ANOVA) test compares knockdowns versus Rbf120a, ***adjusted p value < 5 × 10-4. c ChIP-qPCR performed on larval chromatin using Rbf, E2f1, E2f2 and Dp antibodies at the promoter regions of Ald and HIF1a relative to the negative site (NS). Data are representative of two experimental replicates and show the mean and error bars represent s.e.m., two-way ANOVA test was used, **p value < 0.001. d Molecular mechanism of the metabolic aspect of apoptosis in Rbf mutant cells. The position of the MF is shown by white arrowhead
