Fig. 1

Loss of Dnm2 does not affect division of peroxisomes. a Western blot analysis of HeLa WT, Dnm2^KO, Drp1^KO, and Dnm2^KO/Drp1^KO whole cell lysates. Actin was used as a loading control. b Confocal images of HeLa WT, Drp1^KO, Dnm2^KO, and Dnm2^KO/Drp1^KO cells subjected to immunofluorescence and stained for peroxisomes (Pex14, white). Blue marks the nucleus. An enlargement of the hatched box is shown on the right of each panel. Scale bar = 10 µm. c Computational quantification of average peroxisome length in cell lines. n(WT) = 20 cells, n(Dnm2^KO) = 20 cells, n(Drp1^KO) = 20 cells, n(Dnm2^KO/Drp1^KO) = 18 cells. Data obtained from three independent experiments. Data represents the mean ± S.E.M.; n.s., not significant; ****p < 0.0001. One-way ANOVA with multiple comparisons. d Confocal live-cell image stills of HeLa Drp1^KO and Dnm2^KO/Drp1^KO stably expressing Dendra2-SKL (green) and transfected with mCherry-Dnm2 (red) and/or doxycycline-inducible mScarlet-Drp1 (red) constructs. Enlargements of the hatched boxes depicting peroxisomal morphology in untransfected and transfected cells are shown on the right. Scale Bar = 10 µm and 5 µm (magnifications). e Confocal live-cell image stills of peroxisome fission events in HeLa Dnm2^KO/Drp1^KO cells stably expressing Dendra2-SKL and doxycycline-inducible mScarlet-Drp1. Scale bar = 5 µm