Fig. 3

Loss of Drp1 is dominant to loss of dynamins 1,2,3. a Western blot analysis of MEF DnmTKO^cre, DnmTKO^cre+Drp1^KO control and 4-OHT induced cells on whole cell lysates. Actin was used as a loading control. b Representative confocal image frames depicting mitochondrial morphology and fission events of MEF DnmTKO^cre control and knockout (4-OHT induced) cells. Two enlargements on the right of each panel show fission events over the course of 15 s (red arrowheads). Scale bar = 10 µm. c FRAP analysis of mtDendra2 in MEF DnmTKO^cre, DnmTKO^cre+Drp1^KO control and 4-OHT induced cells. n(DnmTKO^cre) = 23 cells; n(DnmTKO^cre + 4OHT) = 31 cells; n(DnmTKO^cre + Drp1^KO) = 26 cells; n(DnmTKO^cre+Drp1^KO+4OHT) = 23 cells. Data obtained from three independent experiments. Data represents the mean ± S.E.M.; n.s., not significant; *p < 0.05. One-way ANOVA with multiple comparisons. d Number of mitochondrial fission events in MEF DnmTKO^cre, DnmTKO^cre+Drp1^KO control and 4-OHT induced cells counted over a 7.5 min period. n(DnmTKO^cre) = 13 cells; n(DnmTKO^cre+4OHT) = 10 cells; n(DnmTKO^cre + Drp1KO) = 21 cells; n(DnmTKO^cre + Drp1KO + 4OHT) = 20 cells. Data obtained from three independent experiments. Data represents the mean ± S.E.M.; **p < 0.01, ****p < 0.0001. One-way ANOVA with multiple comparisons