Fig. 4

Template characteristics. a Schematic of the membrane template. b Representative images of NAO and Alexa647-labeled streptavidin (SA) fluorescence on the SLB and tubes composed of CL:DOPC (25:75 mol%). Scale bar = 10 µm. c Ratios of NAO and Alexa647-labeled streptavidin (SA) fluorescence on the SLB and tubes composed of CL:DOPC (25:75 mol%); ns, not significant, Mann–Whitney test. n(SLB) = 19 and n(tube) = 25 samplings from a single template preparation. d Schematic showing the expected fluorescence ratios of an externally added GFP-tagged protein that binds the membrane on the SLB, unilamellar and multilamellar tubes. e Representative images showing GFP-LactC2 distribution on the SLB and tubes. Scale bar = 10 µm. f Ratios of GFP-LactC2 and membrane fluorescence on tubes of varying sizes compared to that seen on the SLB; ns, not significant, Mann–Whitney test. n(<50 nm) = 33, n(50–100 nm) = 8, n(100–200 nm) = 2, n(>200 nm) = 1, and n(SLB) = 10 samplings from a single template preparation