Fig. 5

Self-assembly of Drp1. a Representative images of Drp1 ± GFP on the SLB and tubes in the apo (a), GDP-bound (b), and GMP-PNP-bound (c) states. Scale bar = 10 µm. Also shown are ratios of Drp1 ± GFP to membrane fluorescence on the SLB and tubes; ****p < 0.0001, Mann–Whitney test. n(SLB) = 28 and n(tube) = 25 from a single experiment for apo. n(SLB) = 21 and n(tube) = 16 from a single experiment for GDP. n(SLB) = 21 and n(tube) = 15 from a single experiment for GMP-PNP. Yellow arrowheads mark clusters of Drp1 ± GFP on tubes in the GMP-PNP-bound state. d Representative images and associated line profiles showing distribution of Drp1 ± GFP with GMP-PNP. White arrowheads mark clusters of Drp1 ± GFP. e Pearson’s coefficient of correlation between Drp1 ± GFP and membrane fluorescence. n(apo) = 10, n(GDP) = 10, and n(GMP-PNP) = 16 tubes from a single experiment