Fig. 6 | Nature Communications

Fig. 6

From: Dynamin-related protein 1 has membrane constricting and severing abilities sufficient for mitochondrial and peroxisomal fission

Fig. 6

Drp1- and Dnm2-catalyzed membrane fission. a Frames from a time-lapse movie showing Drp1-catalyzed fission. Red arrowheads mark site of fission, yellow arrowheads mark tubes that remain uncut. Scale bar = 10 µm. b Fission probability with Drp1 and Dnm2 as a function of starting tube radius. n(tubes) for each size range is indicated in the plot. c Analysis of Drp1-catalyzed fission with the indicated mutants and orthologs. n(WT) = 10, n(K38A) = 11, n(K557–560A) = 5, n(K569–571A) = 3, n(A395D) = 6, n(Drp S. pombe) = 7, and n(Drp D. melanogaster) = 16 microscope fields of a single experiment where each field displayed 30–35 tubes. d Analysis of Drp1-catalyzed fission on templates of the indicated lipid composition. n(CL mix) = 8 and n(MOM mix) = 7 microscope fields from a single experiment where each field displayed 30–35 tubes

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