Fig. 1
From: Stress-induced formation of cell wall-deficient cells in filamentous actinomycetes
High levels of sucrose affect growth and morphology of K. viridifaciens. a Growth curves of K. viridifaciens in LPB medium supplemented with increasing amounts of sucrose. Values represent the average of five independent replicates. High levels of osmolytes reduce the number and size of colonies (b) in comparison to media without osmolytes (c). Mycelial morphology K. viridifaciens grown in LPB without sucrose (d) and with 0.64 M of sucrose (e). Mycelium was stained with FM5-95 and SYTO-9 to visualize membranes and DNA, respectively. Please note the S-cells (white arrowheads in e) and indentations along the cylindrical part of the hypha, indicated with black arrowheads in the brightfield (BF) section, formed in medium containing high levels of sucrose. Scale bars represent 10 mm (b, c), 20 µm (left panels in d, e) and 10 µm (magnified section in d and e)
