Fig. 3

CIR identify regulatory IL-10-producing CD4⁺ T cells. viSNE analysis of IL-10^pos Foxp3^neg CD4⁺ T cells. Clustering is based on MFI of PD-1, LAG-3, TIGIT, TIM-3, CD49b, and CCR5. Blue circle indicates co-inhibitory receptor rich (CIR rich) region. a Analysis of cells from small intestine, spleen, and lung of aCD3-treated IL-10^eGFP Foxp3^mRFP double reporter mice (n = 4). Data are representative of two independent experiments. b Analysis of untreated IL-10^eGFP Foxp3^mRFP double reporter mice (n = 3). Data are representative of three independent experiments. c Analysis of P. berghei infected IL-10^eGFP Foxp3^mRFP double reporter mice (n = 3). Data are representative of two independent experiments