Fig. 3

PRMT1 depletion causes cardiomyocyte hypertrophy while its overexpression prevents phenylephrine-induced hypertrophy. a Representative immunostaining images of NRVM cells infected with Ad-shscr (adenovirus-sh scrambled RNA) and Ad-shPRMT1 (adenovirus-PRMT1 shRNA). The white dashed line indicates a single cell surface. Scale bar: 50 μm. b Quantification of cell surface area as shown in a. n = 82 (Ad-shscr); n = 70 (Ad-shPRMT1). Data represent means ± SD. ^***P < 0.005, Student’s t-test. c Immunoblot analysis of NRVM/Ad-shscr or NRVM/Ad-shPRMT1 cells. d qRT-PCR analysis of ANP, BNP, and β-MHC expression in NRVM/Ad-shscr and NRVM/Ad-shPRMT1. n = 3. Data represent means ± SD. ^*P < 0.05, ^**P < 0.01, Student’s t-test. e Immunostaining images of PRMT1-Flag transfected NRVM cells (red staining) treated with control or phenylephrine (PE) for 48 h. Scale bar: 50 μm. f Quantification of the surface area of cells similar shown in e. n = 56 (vehicle-pcDNA); n = 54 (vehicle-PRMT1-flag); n = 52 (PE-pcDNA); n = 48 (PE-PRMT1-flag). Data represent means ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, Student’s t-test. g Protein analysis of control or PRMT1-HA transfected NRVM cells treated with vehicle or PE for 48 h for PRMT1 and ANP. h qRT-PCR analysis of ANP, BNP, and β-MHC levels in control or PRMT1-HA transfected NRVM cells treated with vehicle or PE. n = 3. Data represent means ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, Student’s t-test