Fig. 3 | Nature Communications

Fig. 3

From: Temporal control of gene expression by the pioneer factor Zelda through transient interactions in hubs

Fig. 3The alternative text for this image may have been generated using AI.

Transcriptional memory persists in the absence of Zelda. a–h Snapshots of maximum intensity projected Z-stack of Drosophila embryo movies expressing snaE (a–d) or snaE + 3Zld (e–h) at nc13 (a, c, e, g) and nc14 (b, d, f, h), in a white-RNAi (a, b, e, f) or in a zld-RNAi genetic background (c, d, g, h). Nuclei exhibiting a spot of active transcription are false-colored in yellow. i, j Temporal coordination of all activated nuclei in nc14 for snaE (i) and snaE + 3Zld transgene (j), in a white-RNAi (solid curves) and in a zld-RNAi genetic background (dashed curves). Statistics: snaE white-RNAi: 9 movies, n = 972; snaE zld-RNAi: 4 movies n = 156; snaE + 3Zld white-RNAi: 6 movies n = 541; snaE + 3Zld zld-RNAi: 5 movies n = 160. Error bars represent SEM. k, l Kinetics of activation during the first 15 min of nc14 driven by the snaE transgene or the snaE + 3Zld transgene in white-RNAi (solid curves) or in zld-RNAi embryos (dashed curves). The kinetics of nuclei coming from transcriptionally active mothers in nc13 (green curves) is compared to those arising from inactive mothers (red curves). Statistics: snaE white-RNAi: 6 movies, n = 302; snaE zld-RNAi: 4 movies n = 86; snaE + 3Zld white-RNAi: 6 movies n = 273; snaE + 3Zld zld-RNAi: 5 movies n = 111). In Fig. 3, only nuclei located within a 25 μm rectangle centered around the ventral furrow/pseudo-furrow for zld-RNAi are considered. Scale bars represent 10 μm

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