Fig. 1 | Nature Communications

Fig. 1

From: Trisomy silencing by XIST normalizes Down syndrome cell pathogenesis demonstrated for hematopoietic defects in vitro

Fig. 1

System to study the effect of trisomy 21 expression on DS-related pathologies. a Schematic of the inducible XIST RNA-mediated silencing system in Down syndrome iPSCs, in which XIST induces formation of a condensed, heterochromatic chromosome 21 “Barr Body”. b Neural stem cell (rosette) formation after 15 days neural differentiation, with and without induced expression to silence one chromosome 21. The samples treated with dox have significantly more neural stem cells at this time point. Scale bar: 100 μm. c Quantification of the number of neural rosettes at day 15 for two of the isogenic XIST-transgenic subclones. The iPS cells used were from a male, but because regulatory counting elements are removed from the XIST cDNA, this system is also compatible with female cells. Error bars represent s.e.m. from three independent experiments

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