Fig. 2

Trisomy silencing during hematopoietic differentiation. a Schematic of hematopoietic differentiation from DS iPSCs. b Association of H3K27me3 heterochromatic mark with XIST RNA in day 9 purified CD34⁺ differentiated cells treated with dox. Quantification shows over 90% of cells have H3K27me3 marker associated with XIST paints. c RNA FISH of APP and XIST on day 9 differentiating cells. APP RNA transcription foci from all three alleles in untreated cells (top). In treated cells (bottom), only two APP transcription foci are detected, indicating transcriptional silencing induced by XIST expression. Quantification shows over 90% of XIST expressing CD34⁺ cells have only two APP transcription foci (which are not from XIST expressing chromosome). Error bars for b and c represent s.e.m. from three independent scorings