Fig. 4

Amino acids (displayed as dot in Pymol) that involved in the key interactions which identified or discussed in this SINV structure. a The completely or highly conserved amino acids of E1 and E2 that are involved in the interactions of forming the hydrophobic pocket and forming the hydrophobic contact with pocket factor are colored in cyan. The amino acids of E1 that are involved in connecting the stem loop with E1 DIII and E2 domain C are colored in magenta. The completely conserved amino acids of E3 and E2 that are involved in hydrophobic interaction between E3 and β-ribbon connector are colored in green. The completely or highly conserved amino acids of E1 S57, E2 H164 and H211 that form an interaction at neutral pH but become repulsive at low pH to facilitate the dissociation of β-ribbon connector from E1 are colored in blue. The identified glycosylation sites are colored in red. b Amino acids of E1 that are involved in the type I and II inter-triangle interactions are colored in red