Fig. 3

Presentation of antigen by skin-draining DC subsets. a Proliferation and induction of LAP⁺ T cells in the CFSE-low DO11.10 CD4⁺ T cells after co-culture with CD11c⁺ cells isolated from brachial LN of Balb/c mice receiving topical OVA, in the presence or absence of anti-MHCII antibody. b Proliferation and percentage of LAP⁺ T cells in the CFSE-low DO11.10 cells after co-culture with sorted DC subsets (ratio 5:1, Tcells:DCs). N = 4–5 replicates for populations CD103⁺, EpCAM⁺ and CD11b⁺, 1–2 replicates for populations Neg and CD8a⁺. *p < 0.05, **p < 0.01 vs. No DCs. Kruskal–Wallis test with Dunn’s multiple comparisons. Gating for sorting shown in Fig. 2g