Fig. 7

PDL2⁺ DCs from skin-draining lymph nodes present antigen. a Percentage of CFSE_lowDO11.10 CD4⁺ T cells after co-culture with sorted PDL2⁺ (blue) or PDL2⁻ (red) DCs from mice treated with OVA for 48 h. b Representative plots of the expression of LAP, CCR9, CCR6, and CCR4 by CFSE-low DO11.10 CD4⁺ T cells after co-culture with sorted PDL2⁺ DCs. Staining control is shown in grey (fluorescence minus one). c Percentage of CFSE_low LAP⁺, CCR9⁺, CCR6⁺ or CCR4⁺ CD4⁺ T cells after co-culture with sorted PDL2⁺ (blue) or PDL2⁻ (red) DCs from mice treated with OVA patch. N = 2−6 replicates. *p < 0.05, **p < 0.01, ****p < 0.0001 vs. PDL2^-, Two-way Anova, Sidak’s multiple comparison test