Fig. 2
From: MoVE identifies metabolic valves to switch between phenotypic states
Implementation considerations for MoVE strategies. First, a set of static knockouts can be made using canonical genetic engineering techniques. Second, if the growth rate has suffered due to sub-optimality, the strain can be adaptively evolved to maximize growth rate, accumulating genomic modifications. Finally, genetic circuitry can be applied to effect a switch by eliminating flux through target valves, and achieving at least the minimum specific target (e.g., product yield)
