Fig. 3
From: MoVE identifies metabolic valves to switch between phenotypic states
Application of MoVE to a core model for α-ketoglutarate (AKG) production. Line thickness is proportional to pathway flux, metabolites highlighted in blue are biomass precursors. a Wild-type flux distribution of the E. coli core model. Aerobically grown wild-type E. coli is known to partition flux between glycolysis and the pentose phosphate pathway, with a fully active tricarboxylic acid (TCA) cycle and respiratory chain56, leading to maximal biomass yield and growth rate. b Flux-distribution with identified knockouts applied. Following knockout of the predicted reactions and associated genes: pyruvate kinase (PYK, pykAF), succinyl-CoA synthetase (SUCOAS, sucA), glutamate synthase (GLUSy, gltB), and malate dehydrogenase (MDH, mdh), flux is partitioned toward the pentose phosphate pathway from glycolysis, and flux through the TCA cycle is redirected through the glyoxylate shunt, marginally impacting the maximum biomass yield. c Flux-distribution after eliminating flux through knockouts and valves, achieving maximal AKG yield
