Fig. 1 | Nature Communications

Fig. 1

From: Bioconjugation strategy for cell surface labelling with gold nanostructures designed for highly localized pH measurement

Fig. 1

Bioconjugation strategy for cell surface labelling using AuNP. a Explanatory sketch illustrating the structure of the pH nanosensor: the surface of AuNP is functionalized with 4-MBA and HPDP-B. The sulfo-NHS moiety of the biotinylation reagent NHS-B reacts with primary amines in lysines or amino-termini of membrane proteins such as receptors, pores, channels, carriers, pumps, integrins, or enzymes. Two of the four active sites of streptavidin (SA) provide the link between the biotins of NHS-B anchored to proteins and HPDP-B conjugated to AuNP. b Conjugated AuNP labelled with Alexa-SA: the attachment of the nanoparticles to the cell membrane was confirmed using AuNP functionalized with HPDP-B, some biotins of which reacted with Alexa-SA to enable visualization by CFM

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