Fig. 2
Gold nanoparticles distribution on the cell outer membrane surface detected by CFM. The analysis was performed on the cluster of MKN28 cells shown in a and treated with the AuNP of Fig. 1b according to our protocol. In b–f are reported the fluorescence images collected at z = 0, 6, 12, 14, and 16 µm, respectively. Green is the Alexa488 dye linked to the AuNP, while the nuclei were stained with blue Hoechst 33342 dye. In some locations of the glass bottom dish, close to the cell cluster at z = 0, we noticed microscopic AuNP aggregations, in which traces of Hoechst dye were detected. In Supplementary Movie 1, we combined the collected images to create a movie of the z-stack sections through the cells. Scale bar: 10 µm
