Fig. 8 | Nature Communications

Fig. 8

From: Lysosomal protease deficiency or substrate overload induces an oxidative-stress mediated STAT3-dependent pathway of lysosomal homeostasis

Fig. 8The alternative text for this image may have been generated using AI.

Activation of STAT3 in AEP−/− kidney and enhanced hydrolase expression in STAT3c kidneys. a STAT3 and P-STAT3 levels in WT and AEP−/− kidneys. b P-STAT3 detected by IF in WT and AEP−/− kidney sections. Right panel shows quantitation of the number of P-STAT3 positive nuclei per area (n = 35 microscopic fields). (Scale bar = 100μm). Statistical significance was calculated using a two-sided unpaired t-test. c Ki67 and laminin detected by IF in WT and AEP−/− kidney sections. (Scale bar = 20μm). d Similar proteins accumulate in STAT3c and AEP−/− kidney. e Levels of P-STAT3 and CtsD in proximal (PTC) and distal (DTC) tubular cells treated or not with 50 μM MVO26630 overnight. Data are the average ± SD of n = 5 biologically independent samples for PTCs and n = 3 for DTCs. Statistical significance was calculated using a two-sided unpaired t-test

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