Fig. 6

NOD2 signaling in monocytes confers protection of Nlrp12^−/− mice against C. rodentium infection. Bacterial-driven colitis was induced by oral gavage of 1x10⁹ C. rodentium. a Nlrp12^−/− mice (white circles) and wild-type (black circles) controls on days 2, 3, and 4. b CFU counts of C. rodentium-infected Ifit2^−/− (gray circles) and wild-type (black circles) mice on days 3, 5, and 7. c Whole infection course was monitored during 3 weeks by calculating CFU per mL of fecal suspensions from Nlrp12^−/− (n = 7) and Nlrp12^−/−Nod2^−/− (n = 6) mice. d, e Pathogen burden in feces from lethally irradiated wild-type and Nlrp12^−/− recipient mice that were reconstituted with bone-marrows from either wild-type or mutant mice. f Colon lengths, g Representative H&E staining of 5 µm-thick tissue sections from distal colon of Nlrp12^−/− and wild-type mice on day 7 post infection. Scale Bar represents 200 µm. h Inflammatory response of Nlrp12^−/− and control mice to C. rodentium infection was evaluated by calculating crypt lengths from distal colon, i LCN2 protein in feces of infected mice. j Mean proportion of Ly6C^hiMHCII^hi subset as a percentage of live Ccr2-expressing colonic CD11b⁺ cells from the lamina propria of chimeric mice. k Mean proportion of inflammatory monocytes as a percentage of live MHCII-expressing colonic CD11b⁺ cells from the lamina propria of wild-type and mutant mice. l Representative expression of CD11c and CD64 expression amongst total live fraction of MHCII-expressing colonic CD11b⁺ cells from Nlrp12^−/−, Nod2^−/− Nlrp12^−/− and wild-type mice. Statistical significance was assessed by non-parametric Mann–Whitney test. P < 0.05 (*) and P < 0.01 (**) were considered statistically significant. Data represent mean ± SEM