Fig. 1

Deletion of IgSF9b normalizes anxiety-related behavior in Nlgn2 KO mice. a, b WT, Nlgn2 KO, IgSF9b KO, and double KO mice were assessed for anxiety-related behavior in an OF test. c Representative tracks of OF exploration. d Time spent in the anxiogenic center of the OF. e Distance traveled in the center of the OF, expressed as percentage of total distance traveled. f Number of entries into the center of the OF. g Total distance traveled in the OF. h Mice were assessed for anxiety-related behavior in EPM. i Representative tracks of EPM exploration. j Time spent in the anxiogenic open arms of the EPM. k Distance traveled in the open arms of the EPM, expressed as percentage of total distance traveled. l Number of entries into the open arms of the EPM. m Total distance traveled in the EPM. Statistically significant ANOVA comparisons are marked in gray at the top of the panels and are listed in Table 1. For all other ANOVA comparisons, F < 1. Post-hoc analysis: *p < 0.05 relative to WT, **p < 0.01 relative to WT, ***p < 0.001 relative to WT, ^#p < 0.05 relative to double KO, ^##p < 0.01 relative to double KO, ^###p < 0.001 relative to double KO. Error bars represent SEM, n = 10–12 mice per genotype for OF, n = 9–14 mice per genotype for EPM