Fig. 5
Dynamic regulation of genes and bioprocesses at birth. a RNA-seq samples were collected from seven time points of mouse lung development. b Top panel: six representative temporal gene expression patterns were identified; red dashed lines indicate PND1. Bottom panel: the significance (p-value), gene counts, and transcription factors in the six representative gene expression patterns. c Functional annotations uniquely or commonly enriched by the temporal gene expression patterns “Pattern 46” and “Pattern 47”. d, e The dynamic patterns of representative genes in four biological processes during mouse lung development. Red lines represent the data from mouse lung at PND1. The four biological processes and representative genes include surfactant synthesis (Sftpb, Sftpc, Abca3, Sftpd, Lpcat1), fluid transport and clearance (Scnn1a, Scnn1b, Scnn1g, Cftr, Slc6a14), cell proliferation (Ccna2, Ccnb1, Cdk1, Cdk2, Mki67), and response to oxidative stress (Cat, Fos, Gclc, Mapk14, Nfe2l2). d Gene expression patterns derived from RNA-seq data of mouse lung at E16.5-PND28. e Gene expression patterns derived from published RNA microarray data of mouse lung development from E9.5 to PND5610. Gray dots represent individual data points. Black lines represent fitted locally weighted scatterplot smoothing profiles; gray regions are the confidence intervals around smoothing
