Fig. 6
UPR activity in the mouse lung at birth. a Associations of unfolded protein response (UPR) gene expression and specific cell types based on the single cell Drop-seq data. Associations (nodes) with Fisher’s exact test p-value < 0.05 and enrichment score ≥ 1.5 are shown. Node size is proportional to –log2-transformed Fisher’s exact test p-value. Node color gradient is proportional to the enrichment score. Sensor stress sensors, TF transcription factors, ERAD endoplasmic reticulum-associated degradation, Lipid lipid biosynthesis. b Whole lung lysates from three mice at each age of E18.5, PND1, and PND7 were immunoblotted for ATF6, SYVN1, CHOP, and β-actin. c Quantification of immunoblotting after normalization to β-actin. d Whole lung RNA was used to quantitate Scap, Cebpd, and Srebf2 RNA from three mice at each age of E18.5, PND1, and PND7. e ATF6 staining was increased in airway of PND1 compared to E18.5 and E16.5 tissue. Scale bar is 50 μm. Images are representative of at least three embryos/animals for each time point. Boxed regions are zoomed in. In c and d, *p-value < 0.05 from E18.5 determined by one-way ANOVA with Dunnett’s multiple comparison, mean ± S.E.M. Source data are provided as a Source Data file
