Fig. 5

Effect of the rs7665090^G variant on MS lesion pathology. a, b Bright-field images of perivascular infiltrates within chronic active MS lesions labeled with anti-CD3 antibody. Scale bar = 50 µm. c, d Quantification of infiltrating, perivascular CD3⁺ T cells per blood vessel in the rim (c) and center (d) of 29 lesions (15 risk group, 14 protective group) from 10 MS cases (5 cases per group) shows a significant difference between groups in the rim (p = 0.0413), but not in the center (p = 0.1943). Dots represent average CD3⁺ T cells per blood vessel for all lesions of a single case. The number of lesions from each case ranged from 1 to 5. e Ratios of perivascular CD3⁺ T cells to CD68⁺ microglia in the rim show a significant difference between groups (p = 0.0199). f Quantification of lesion sizes, determined as area of demyelination, shows a significant difference between groups (p = 0.0491). Dots represent average size for all lesions of a single case. Data represents mean ± s.d. p-Values shown for the unpaired t-test. *p < 0.05. g Heatmap of computed Pearson correlation coefficients from expression values of 5 cases per group (red, p-value < 0.05; blue, p-value > 0.05). h Correlation of NF-κB p65 nuclear expression with CCL5 (r = 0.88, p = 0.001) and CXCL10 (r = 0.84, p = 0.003) expression in astrocytes. i Correlation of infiltrating CD3⁺ T cell with cytosolic NF-κB p50 (r = 0.80, p = 0.005) and CXCL10 (r = 0.67, p = 0.036) expression. j Correlation of lesion size with IL-15 (r = 0.88, p = 0.001) and C3d (r = 0.81, p = 0.005)