Fig. 3
Some microRNAs are altered by miR-122 removal, but these are largely in imprinted loci. a Reads per million mapped microRNAs (RPM) values for all selected microRNAs at various timepoints after Cre-mediated miR-122 removal including miR-127 at the chr12qF1 Dlk1-Dio3 locus and miR-483 at the Igf2 locus (n = 3 for days 0, 7, 14, 21, 45, and 200; n = 1 for remaining timepoints). b Small RNA northern blot of miR-376a, present on the chr12qF1 locus at various points following miR-122 excision. c Scatterplot of mean expression per microRNA for small RNA-seq RPM values in miR-122–/– mouse livers relative to wild-type mouse livers. MicroRNAs that map to the chr12qF1 microRNA locus are highlighted in red while those at the Igf2 locus are blue (n = 13 for miR-122–/– and n = 20 for WT). d Comparison of mean expression, expressed as a percentage of total microRNA reads, for miR-21 and the combined normalized read counts for all microRNAs at the chr12qF1 locus (n = 13 for miR-122–/– and n = 28 for WT). e Levels of chr12qF1 microRNAs after delivery of an shRNA expressing Mir122 or a control shRNA in miR-122–/– mouse livers (n = 7 mouse livers for 122−/− and 122−/− + U6-sh-Scr, n = 3 mouse livers for 122−/− + U6-sh-miR-122). f Expression of miR-122 and all cumulative reads mapping to chr12qF1 microRNAs at various timepoints during mouse development. g Expression of miR-122 and all cumulative reads mapping to chr12qF1 microRNAs at various timepoints following Cre-mediated miR-122 excision (n = 3 for days 0, 7, 14, 21, 45, and 200; n = 1 for remaining timepoints). h Expression of chr12qF1 and Igf2 locus microRNAs at various timepoints in miR-122–/– mouse livers
