Fig. 1
From: The gut microbiome is required for full protection against acute arsenic toxicity in mouse models
Arsenic levels in feces and host tissues of wild-type C57BL/6 mice. Cefoperazone (Cef) and Sham treatments were initiated 48 h prior to inorganic sodium arsenate (iAsV) exposure in drinking water at either 25 ppm (left) or 100 ppm (right). For 25 ppm exposures, a total of 20 mice were used in two replicate experiments (n = 5 mice per treatment group; tissue from one mouse in Sham group was lost during processing). For 100 ppm exposures (right), a total of 10 mice were used to examine fecal excretion (n = 5, Sham; n = 5, Cef) and an additional (replicate) experiment was added for tissue bioaccumulation (an additional 8 mice; n = 5, Sham; n = 3, Cef). Group-wise means are shown in all graphs (scatter plots and histograms) and error bars represent 95% confidence interval of the mean. a Significantly lower levels of arsenic were excreted in feces of Cef-treated mice beginning 24 h after exposure at both doses (p < 0.0001, two-way ANOVA after log transformation). b Significantly higher levels of arsenic accumulated in organs of Cef-treated mice after 14 days of exposure (25 ppm, p < 0.0001; 100 ppm, p = 0.0003, two-way ANOVA after square root transformation) with the greatest differences in lung (25 ppm) and liver (100 ppm). p-Values in b were adjusted for multiple comparisons (Sidak’s multiple comparisons test)
