Fig. 2
Electron transfer and membrane potential generation. a Absorbance changes at 552 nm after mixing reduced TpIc3 with empty liposomes (black), Qrc proteoliposomes (without MK4) (magenta), MK4-liposomes (blue), and Qrc/MK4 proteoliposomes (green). b Kinetic trace subtraction (green circles) of MK4-liposomes data (blue) from that of Qrc/MK4 proteoliposomes (green), fitted with a single exponential (black line) with equation: (−0.12 ± 0.01) × exp(0.65±0.05)×t + (0.40 ± 0.03), where ± gives the standard deviation error obtained from the fit for each value. c Changes in A640 nm − A612 nm after mixing reduced TpIc3 with liposomes in the presence of oxonol V. The absorbance presented is the subtraction between liposomes without and with valinomycin (traces used for the subtractions are presented in Supplementary Fig. 6): empty liposomes (black), Qrc proteoliposomes (magenta), MK4-liposomes (blue), and Qrc/MK4 proteoliposomes (green). All traces correspond to the average of at least seven experiments performed with at least two distinct liposome preparations
