Fig. 3

Electron and proton transfer. a Absorbance changes at 552 nm and b at 560 nm, after mixing reduced TpIc₃ with valinomycin permeabilised liposomes prepared with phenol red in the inner solution. Empty liposomes (black), Qrc proteoliposomes (magenta), MK4-liposomes (blue), and Qrc/MK4 proteoliposomes (green). c Absorbance changes at 552 nm and d at 560 nm, after mixing reduced TpIc₃ with valinomycin permeabilised liposomes and phenol red in the outer solution, MK4-liposomes (blue) and Qrc/MK4 proteoliposomes (green). e Absorbance changes at 552 nm and f at 560 nm, after mixing reduced TpIc₃ with valinomycin permeabilised liposomes prepared with phenol red in the inner solution in the presence of CCCP, MK4-liposomes (blue), Qrc/MK4 proteoliposomes (green), CCCP permeabilised MK4-liposomes (grey), and CCCP permeabilised Qrc/MK4 proteoliposomes (black). All traces correspond to the average of at least seven experiments performed with at least two distinct liposome preparations