Table 1 Guide RNAs and primer sequences used for CRISPR-Cas9 gene editing
From: The mTORC1/4E-BP1 axis represents a critical signaling node during fibrogenesis
RICTOR, exon 29 | |
|---|---|
gRNA | AATATCGGCTCATCAAATTGGGG |
MiSeq forward primer | ACACTCTTTCCCTACACGACGctcttccgatctATGACCTACCCTCTGATGGAAAG |
MiSeq reverse primer | TGACTGGAGTTCAGACGTGTGctcttccgatctTTTTTCTCTCTCAGAGATGAGGT |
RAPTR, exon 26 | |
|---|---|
gRNA | CCGCGTCTACGACACAGAAGGATGG |
MiSeq forward primer | ACACTCTTTCCCTACACGACGctcttccgatctACCCAACCAAATGGCAGTGACAC |
MiSeq reverse primer | TGACTGGAGTTCAGACGTGTctcttccgatctTGCCTGTGTTTGGCTCTAGGACA |
