Fig. 5

UroA/UAS03 prevent TNBS-induced colitis and sustain beneficial barrier activities. a Pre-TNBS treatment. Male C57BL/6 mice (n = 5 per group at 7–8 week old age) were given orally vehicle (Veh; 0.25% sodium carboxymethylcellulose) or UroA or UAS03 (20 mg/kg/bodyweight) daily for one week followed by rectal administration of TNBS to induce colitis. These mice did not receive any treatment post-TNBS administration. Mice were euthanized 72 h post-TNBS administration and characterized. b Post-TNBS treatment. Another set group of C57BL/6 mice (n = 5 per group at 7–8 week old age) received Veh or UroA or UAS03 (20 mg/kg) 24, 48, and 72 h post-TNBS. c Percent body weight loss was recorded after TNBS-administration. (No TNBS- Solid black line; Veh + TNBS- Solid red line; Pre-TNBS + UroA- Solid blue line; Pre-TNBS + UAS03- solid purple line; Post-TNBS + UroA- dashed blue line; Post-TNBS + UAS03- dashed purple line). d Representative colon images of control (no TNBS) along with vehicle/UroA/UAS03 treated mice from pre- and post-treatment groups. e Ratio of colon weight/length, f intestinal permeability was evaluated using FITC-dextran leakage assay. g Serum levels of IL-6 and TNF-α were measured using standard ELISA methods. Statistical analysis was performed (unpaired t-test) using Graphpad Prism software. Error bars, ±SEM ***p < 0.001. Source Data are provided as a Source Data File