Fig. 4

CKIP-1 is highly expressed in atherosclerotic lesion. a mRNA levels in pMΦ for CD68, CKIP-1, and ABCA1 (control gene) were compared between chow-fed and Western diet-fed Apoe^−/− mice. b CKIP-1 expression was detected by immunofluorescence staining and located with CD68 in Apoe^−/− mouse atherosclerotic lesions (CKIP-1, green; CD68, red; DAPI, blue). Scale bar, 500 μm. c Immunofluorescence staining for CKIP-1 and REGγ in Apoe^−/− mouse atherosclerotic lesions (CKIP-1, green; REGγ, red; DAPI, blue). Scale bar, 400 μm. d CKIP-1 mRNA levels were compared between the aortic arches of chow-fed WT mice and Apoe^−/− mice, and between the aortic arches of Apoe^−/− mice fed chow or a Western diet. e CKIP-1 mRNA levels were compared between the aortic arches of chow-fed WT mice and Ldlr^−/− mice, and between the aortic arches of Ldlr^−/− mice fed chow or a Western diet. f Atherosclerotic lesions of chow-fed and western-diet-fed Apoe^−/− mice were stained by Oil Red O. CKIP-1 expression in aortic arch of each group was detected by western blot. g Immunohistochemical staining for CKIP-1 in human autopsy atherosclerotic plaques, also stained with H&E to show histological features of the plaque. Scale bar, 50 μm. Data represent mean ± s.e.m. of n = 3 biologically independent experiments (a, d, e). P values were calculated by two-tailed Student’s t-test (a, d, e). *P < 0.05, **P < 0.01, ***P < 0.001. The precise P value and statistics source data are in Supplementary Data 2. Unprocessed original scans of blots are shown in Supplementary Fig. 6