Fig. 3

CRISPRi screening with Dolcetto. a Comparison of sgRNA activity as a function of distance from the FANTOM-annotated transcription start site (TSS). Vertical dotted lines indicate the preferred targeting site. b Schematic of viability screens performed with Dolcetto in two cell lines. c dAUC comparison of sgRNA-level performance across CRISPRi libraries by cell line. dAUC values of individual replicates are plotted as X’s while dAUCs calculated using the average log2-fold change of replicates are plotted as circular points. For hCRISPR-v2 Round 1 only, the dAUC of the hCRISPR-v2 sgRNAs that would be picked in round 1 of our selection heuristic is plotted. Brunello dAUC values are plotted for comparison. d ROC-AUC comparison of gene-level performance across CRISPRi libraries by cell line. ROC-AUC values of individual replicates are plotted as X’s while ROC-AUCs calculated using the average log2-fold change of replicates are plotted as circular points. Brunello ROC-AUC values are plotted for comparison. e Subsampling analysis, calculating the ROC-AUC of n sgRNAs per gene in Dolcetto and hCRISPRi-v2. Error bars represent s.d. calculated from different iterations of library sampling. f Fraction of sgRNAs selected in each round using the heuristics described in Supplementary Table 2 for Dolcetto and hCRISPRi-v2