Fig. 3

Affinity Reagents for Chemoproteomics. a Volcano plot of MTM7172-enriched proteome (labeled blue) from HEK293 cell lysate, with targets significantly (FDR=0.05, S0 = 0.2) competed by 20 μM MS023 with respect to 20 μM MS094 negative control. Proteins marked in purple indicate known interactors of the identified direct targets of MS023 close to the significance threshold. b Volcano plot of (A-395)-NH₂-enriched proteome (labeled blue) from G401 cell lysate, with targets significantly (FDR = 0.05, S0 = 0.2) competed by 20 μM A-395 with respect to 20 μM A-395N negative control. c Volcano plot of SGC2077-enriched proteome (labeled blue) from Jurkat cell lysate, with targets significantly (FDR = 0.05, S0 = 0.2) competed by 20 µM SGC0946, with respect to DMSO control. d STRING network evaluation of targets significantly competed by MS023. Lines in the STRING evaluation represent evidenced interactions, with line thickness indicating confidence (high to low). e STRING network evaluation of targets significantly competed by A-395. The chemical structures of the chemical biology reagents, MTM7172, (A-395)-NH₂, and SGC2077, are shown in Supplementary Table 2. See also Supplementary Fig. 1