Fig. 2
Lineage tracing reveals the kinetics of the endothelial hierarchy in tumors. a Schematic diagram of the Cdh5-CreER RosaYFP lineage tracing model employed with B16-F0 melanoma cells injected at day 0 (D0), with mice receiving tamoxifen (Tam) 3-days post tumor inoculation. Flow cytometry plots demonstrate that D5 post tumor inoculation, only EVP and TA amongst CD34+ LIN-YFP+ cells can be observed. At D10 EVP are absent and by D15 only mature D cells can be observed (n = 5). b Immunofluorescence at D5 shows individual YFP+ foci. At D10 and 15 entire YFP+ vessel structures can be observed. c Schematic diagram of the Sox18-CreER RosaYFP lineage tracing model employed with B16-F0 melanoma cells injected at day 0 (D0), with mice receiving tamoxifen (Tam) 3-days post tumor inoculation. Flow cytometry plots demonstrate that D5 post tumor inoculation, only EVP and TA amongst CD34+ LIN-YFP+ cells can be observed. At D10 EVP are absent and by D15 only mature D cells can be observed (n = 5). d Immunofluorescence at D5 shows individual YFP+ foci. At D10 and 15 entire YFP+ vessel structures can be observed. Scale bar represents 50 and 150 μm, respectively. Results presented as mean ± SEM. EVP endovascular progenitor, TA transit amplifying, D definitive differentiated
