Fig. 1 | Nature Communications

Fig. 1

From: Online photochemical derivatization enables comprehensive mass spectrometric analysis of unsaturated phospholipid isomers

Fig. 1

Schematic of the LC–PB–MS/MS system for lipid identification at C=C location level. a The key components include lipid class separation on a HILIC column employing acetone/ACN/NH4Ac (10 mM) aqueous solution as mobile phase, a flow microreactor made from FEP tubing and a low-pressure mercury lamp (emission at 254 nm wavelength) for online coupling with LC and ESI–MS, and formation of C=C diagnostic ions via CID of the PB products of unsaturated GPs (PB–MS/MS). b The analysis workflow consists of two conventional LC–MS/MS runs for lipid identification at fatty acyl level and data dependent LC–PB–MS/MS for lipid identification at C=C location level. Data analysis was conducted using a home-developed program, Lipid Omega Analyzer

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