Fig. 4
Analysis of GPs containing C18:1 Δ9/Δ11 isomers in human plasma samples. a Hierarchical cluster analysis of IΔ9/IΔ11 in normal (N1–N6) and T2D plasma samples (T2D1–T2D6) provides correct grouping of the two sets of samples. Colors represent different ratios as indicated by the color bar. b Seven pairs of lipid isomers (C18:1 Δ9/Δ11) are found to exhibit significant changes in relative composition ratios between normal control and T2D. Differences between the two groups were evaluated using the two-tailed student’s t test (***P < 0.001). Each value represents the mean ± s.d. (N = 6). c, d Comparisons of individual-to-individual variations based on c relative quantitation (I/IIS) of PE 16:0_18:1 and d relative isomer ratio (IΔ9/IΔ11) measurement of PE 16:0_18:1 in normal and T2D plasma samples. Each value represents the mean ± s.d. (N = 3). Precision of relative isomer ratio measurements is much less affected by individual-to-individual variations as compared to relative quantitation measurements at subclass level
